Method based on electrophoresis and gel extraction for obtaining genomic DNA-free cDNA without DNase treatment.
نویسندگان
چکیده
منابع مشابه
Total RNA was extracted using the TRIzol reagent (Invitrogen) according to manufacturer’s instructions, whereas from caryopses
Total RNA was extracted using the TRIzol reagent (Invitrogen) according to manufacturer’s instructions, whereas from caryopses it was isolated by a LiCl based method. The resulting RNA was treated with RNase-free DNase I (Promega) according to the manufacturer’s protocol. Following digestion, nucleotides were removed from RNA using a G50 sepharose buffer exchange column (Amersham). Absence of g...
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عنوان ژورنال:
- BioTechniques
دوره 37 5 شماره
صفحات -
تاریخ انتشار 2004